Study on Development of Mouse Preimplantation Embryos in Culture Media with Different Composition of Energy Sources / 대한산부인과학회잡지

OBJECTIVE: The objective of this study was to examine the effect on development of mouse preimplantation embryos in culture media with different composition of energy sources in vitro culture. METHODS: Two hundred and seventy one two-cell embryos were cultured in four different culture system for 96 hours. Group I (n=61) was cultured in DMEM-G (DMEM with glutamine) only, groupII (n=64) was cultured in DMEM-GGP (DMEM with glutamine, glucose and pyruvate) only, group III (n=72) was cultured for 48 hours in DMEM-G and then transferred to DMEM-GGP and group IV (n=74) was cultured for 48 hours in DMEM-GGP and then transferred to DMEM-G. Development of embryos in each group was observed every 24 hours. RESULTS: After 24 hours, the rate of development > or = 3-cell was significantly higher in groupII (87.5%) and IV (86.5%) compared with group I (59.0%) and III (62.5%). After 48 hours, the rate of development into > or = morula stage was significantly higher in GroupII (79.7%) and IV (86.5%) compared with group I (34.4%) and III (37.5%). After 72 hours, the rate of development into blastocyst was significantly higher in group IV (74.3%) compared with group I (49.2%) and III (45.8%). After 96 hours, the rate of development into > or = expanded blastocyst was significantly higher in group IV (70.3%) compared with group I (32.8%),II (53.1%), and group III (40.3%). CONCLUSION: Mouse preimplantation embryos development was the most effective in culture system with DMEM-GGP for 48 hours and then transferred to DMEM-G.

Angiogenesis and p53 Protein Expression in Squamous Cell Lesions of the Uterine Cervix / 대한부인종양콜포스코피학회잡지

OBJECTIVE: Tumor angiogenesis is essential for the progression and metastases of solid tumors. Microvessel density(MVD), a measure of tumor angiogenesis, has a prognostic significance in many types of tumors for predicting metastasis and survival. This study had two primary goals: 1. To determine how MVD correlates with tumor invasion in early squamous cell carcinoma(SCC) of the uterine cervix, and 2. To compare p53 protein expression in normal tissue with squamous cell lesions of the uterine cervix. METHODS: Quantification of MVD was performed on 59 specimens of cervical squamous cell lesions by immunohistochemical staining for factor VIII-related antigen. MVD was counted in a x200 field in the most active area of neovascularization. The same tumor sections were also immunohistochemically stains for p53 protein. RESULTS: 1. Compared with nonneoplastic areas, the degree of MVD was significantly increased in low- grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and squamous cell carcinomas(p=0.045). Microinvasive squamous cell carcinoma showed the highest MVD value. 2. p53 protein expression was increased in greater severity cases compared with mild cases(p=0.000). CONCLUSION: In the uterine cervix, MVD was significantly increased in microinvasive squamous cell carcinoma. p53 protein expression was well correlated with progression of squamous cell lesions.

The treatment of cervical pregnancy in Korean / 대한산부인과학회잡지

OBJECTIVE: The study was designed to find out current state of conservative treatment modalities for cervical pregnancies in korean. METHOD: 64 cases of cervical pregnancies reported by the joining of The Korean Society of Obstetrics & Gynecology from 1960 to 2000 and the 10 cases at Chungnam National University Hospital were analyzed retrospectively. RESULTS: The average age of the 74 cases was 31.7+/-6.6 years, and the average gestational age at the time of diagnosis was 8.3+/-3.4 weeks. The average duration of hospitalization was 8.2+/-4.8 days. Most (67/74) of the patients had previously experienced delivery or abortion. Out of the 74 cases, 36 had total hysterectomy; 4 cases had curettage; 12 were used only methotrexate; 11 had combined curettage and methotrexate; 10 cases had Foley catheter ballooning after curettage. From the 1960's to the end of 1980's, 33 (84.6%) cases out of 39 were performed hysterectomy. Whereas mainly conservative methods, in particular methotrexate treatment, were performed in the 1990's. Among the conservative modalities, Foley catheter ballooning after curettage had the shortest length of hospitalization with 4.3 days. CONCLUSION: After comparing the conservative methods for treating cervical pregnancies, it was found that methotrexate treatment was performed the most; however, Foley catheter ballooning after curettage seems to be an excellent treatment option.

Primary Ovarian Transitional Cell Carcinoma / 대한산부인과학회잡지

Primary ovarian transitional cell carcinoma is extremely rare tumor. The histologic subtype was divided from malignant Brenner tumor due to it's own histologic characteristics and chemosensitive nature. Most of recent studies revealed that transitional cell carcinoma has a good response to chemotherapy and long-term survival. Recent histopathologic reports show that transitional cell carcinoma of the bladder and of the ovary are immunophenotypically different. We experienced a case of primary ovarian transitional cell carcinoma, and report this case with a brief review of the concerned literatures.